NanoMesureFrance WEBINAR

Pr. Hervé COTTET (Univ. MONTPELLIER / FR) and Yasmine SEBTI (NanoScale-MetriX / FR) will present a webinar entitled "Taylor or not Taylor for sizing in nanosciences".

Taylor dispersion analysis (TDA) is a new promising technique for the determination of diffusion coefficients and hydrodynamic radius of nanoscale objects. The principle of this method is based on the band broadening of a solute plug injected in a miniaturized Poiseuille flow. It allows determining the hydrodynamic radius of virtually any mixture of solutes, on a range of size ranking between 0.1 and 300 nm.

In this presentation, the principle of the method, its advantages and limitations will be presented. TDA is insensitive to the presence of dusts (contrary to scattering techniques), and leads to a accurate size distribution of the sample generally based on the weight-average of the constituents. With very small injected volumes (nL), application to ultra-small nanoparticles (below 5 nm), straightforward implementation, the absence of size-calibration, no filtration of the sample, TDA is a method of choice for the size characterization of solutes in a myriad of applications.

Different examples will be presented on various pharmaceutical / biomedical topics including, mRNA loaded lipidic nanoparticles and their formulations (LNP), proteins and vaccine antigens, drug delivery nanoparticles or nanogels, cubosomes and liposomes, microemulsions, beta-amyloïd peptide mixtures and their aggregates, and antigen/adjuvant interactions in vaccines.