Dr. Christophe ZIMMER - SMLM with and without deep learning

abbelight

In this webinar you will see the differences between using SMLM with and without deep learning.  Moreover, PhD Zimmer will talk about bio.

C. Zimmer is a computational biophysicist. He holds a PhD in astrophysics from University Paris 11 and did a postdoc in space physics at UCLA (USA). He then moved to Institut Pasteur (Paris) for a second postdoc on image processing for biology. Since 2008, he heads the Imaging and Modeling Unit, which develops imaging and modeling approaches for biomedical research. Since mid-2020, he also heads the Computational Biology Department of Institut Pasteur.Abstract:Single molecule localization microscopy (SMLM) has matured into a powerful and widely used super-resolution imaging method. This talk will highlight recent and ongoing developments of our lab to address three challenges of SMLM by computation.One challenge is 3D super-resolution imaging of entire cells. We present ZOLA-3D, a combined optical and computational method that enables versatile 3D super-resolution imaging over up to roughly 5 micrometer depth and was recently adapted to dual objective microscopy.A second challenge is to visualize cells at high resolution and with high throughput. SMLM delivers exquisite spatial resolution, but at the price of very low throughput. We present ANNA-PALM, a technique based on deep learning that after adequate training can reconstruct high resolution views from strongly under-sampled SMLM data, enabling considerable speed-ups without compromising spatial resolution.A third challenge is to use and reuse SMLM data obtained by the community. We will highlight shareloc, an online platform designed to facilitate the sharing and reanalysis of SMLM data and illustrate its benefit by increased robustness of ANNA-PALM.Finally, time permitting, we will also present Imjoy, a computational platform designed to facilitate the uptake of deep learning methods in the community. Image of actin from Dr. Christophe Zimmer's laboratory.

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Dr. Christophe ZIMMER - SMLM with and without deep learning

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Dr. Christophe ZIMMER - SMLM with and without deep learning

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